Abstract
Summary. Data are presented which show the extent of fragmentation found in Immune Serum Globulin (ISG) stored for various intervals of time at 2°C. As an assay of fragmentation in ISG preparations, gel filtration analysis was found to give results which correlated well with those obtained by ultracentrifugation.The pH at which the fraction III is separated in the cold ethanol fractionation process, Method 9, has been shown to be important in determining the stability of the fraction II separated subsequently. When fraction III was precipitated at pH 5.4 instead of pH 5.15‐5.2 the fraction II separated subsequently under the usual conditions yielded ISG solutions which were stable at 2°C. The antibody in this preparation appeared to be normal in quality but reduced in quantity by about 15%. Removal of the fraction III at pH values below pH 5.2 allowed the separation of fraction II which yielded ISG solutions which readily underwent fragmentation during storage.
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