Abstract
The effects of several fixatives on the PRL receptorhave been examined using slices and particulate fractions from pregnant rat and rabbit liver and rabbit mammary glands. After fixation using picric acid-formaldehyde, the binding affinity for ovine PRL (oPRL) was not substantially affected, while the capacity of the oPRL receptor was reduced from 689 to 420 fmol/mg in the rat liver and from 212 to 143 fmol/mg in the rabbit liver membrane fraction. Similarly, fixation using Lillies' buffered formalin reduced the binding capacity of the oPRL receptor in the rat liver to 410 fmol/mg and, in the rabbit liver membrane fraction, to 153 fmol/mg. Fixation of the 100,000 × g pellet using 10% (vol/vol) unbuffered formaldehyde and 4% paraformaldehyde plus 5% glutaraldehyde destroyed specific oPRL-binding sites, and the latter fixative increased nonspecific binding significantly (P < 0.05). Other fixatives gave intermediate results. Direct exposure of the 100,000 × g pellets to 5% acetic acid in ethanol and 7% forma...
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