Stability of Haskap Berry (Lonicera Caerulea L.) Anthocyanins at Different Storage and Processing Conditions

Rabie Khattab,Marianne Su-Ling Brooks,Amyl Ghanem

doi:10.5539/jfr.v5n6p67

Rabie Khattab, Marianne Su-Ling Brooks + Show 1 more

Open Access

https://doi.org/10.5539/jfr.v5n6p67

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Abstract

The effect of freezing, frozen storage (–18 °C for 6 months), thawing, juice extraction, and hot-air drying on the anthocyanin profile of haskap berry (Lonicera caerulea L.) was investigated using RP-HPLC. Five anthocyanins (ANCs) were quantified: cyanidin 3,5-di-glucoside (4.27 % of the total ANCs), cyanidin 3-glucoside (89.39 %), cyanidin 3-rutinoside (2.07 %), pelargonidin 3-glucoside (0.83 %), and peonidin 3-O-glucoside (3.44 %). Freezing did not significantly affect the content of individual ANCs, while frozen storage resulted in significant reductions (16.00-24.50 %). Thawing the frozen berries in the microwave oven retained the highest content of different ANCs. The highest degradation, however, occurred while thawing at room temperature. Extracting juice from the berries significantly reduced the content of individual ANCs. Drying the berries to 25 % moisture content at 60, 100, and 140 ºC reduced the individual ANCs by 73.85-76.19, 78.46-80.95 and 90.77-95.40 %, respectively. The overall stability of the five ANCs during storage and processing is summarized by the following trend (from most to least stable): peonidin 3-O-glucoside > pelargonidin 3-glucoside > cyanidin 3,5-diglucoside > cyanidin 3-rutinoside > cyanidin 3-glucoside.

Highlights

The haskap berry (Lonicera caerulea L.) has been recently introduced as a commercial crop to the North American market
Five ANCs were identified in the whole fruit including cyanidin 3,5-di-glucoside (4.27 % of the total anthocyanin content (TAC)), cyanidin 3-glucoside or C-3-G (89.39 %), cyanidin 3-rutinoside (2.07 %), pelargonidin 3-glucoside (0.83 %), and peonidin 3-O-glucoside (3.44 %)
These results are in agreement with those reported by Chaovanalikit et al (2004) and Andersen & Jordheim (2007) where C-3-G was reported to dominate in berries of most Lonicera species

Summary

Introduction

The haskap berry (Lonicera caerulea L.) has been recently introduced as a commercial crop to the North American market. The total anthocyanin content (TAC) of haskap berry was found to be up to 13.00 mg cyanidin 3-glucoside (C-3-G) equivalents per g fresh weight (FW) (Bakowska et al, 2007; Fan et al, 2011; Rupasinghe et al, 2012). This berry has much higher antioxidant potential than that reported for blueberry, blackberry, raspberry, bilberry, strawberry, sea buckthorn and black currant (Rop et al, 2011; Raudsepp et al, 2013; Celli et al, 2014)

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