Abstract
Two different hair reference materials, one produced from authentic hair displaying an ethyl glucuronide (EtG) content of about 25pg/mg and one obtained by fortification of blank hair to an EtG level of 85pg/mg were submitted to accelerated aging between 4°C and 60°C for periods between one and 24 months. Subsequently, the EtG content was determined in the aged samples and untreated reference samples stored at −22°C under repeatability conditions following the so-called isochronous approach. The EtG content remained stable even at 40°C for 24 months and at 60°C over six months. This is in contrast to many organic analytes contained in trace concentrations in diverse matrices. A slight but significant increase of the recovered EtG in case of authentic hair samples having been exposed for 24 months between 4°C and 60°C may be due to a temperature-driven process that allows increased recoveries of the physiologically embedded EtG.
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