Abstract
The suitability of the broad-host range vectors pRK290, pVK100 and pLAFRI respectively for use with the photosynthetic purple non-sulphur bacterium, Rhodospirillum rubrum has been examined. All vectors are RK2 (RP4) derivatives (incompatibility group P-1) and contain the mob region of RK2. The three vectors could each be transferred from Escherichia coli by triparental mating in the presence of a second E. coli donor containing the helper plasmid pRK2013. The conjugation frequency was approx. 10 −1 in all cases. Selection of transconjugants was performed in the presence of tetracycline (pRK290, pLAFRI, pVK100) or kanamycin (pVK100). Southern-blotting experiments after many generations showed no rearrangements to have occurred. In the absence of antibiotic selection all three vectors were maintained for at least 15 generations under aerobic chemotrophic conditions but were rapidly lost when the culture was grown phototrophically (anaerobic).
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