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Abstract
Background3-Bromotyrosine (3-BrY) is a stable product of eosinophil peroxidase and may serve as a marker of eosinophil activation. A gas chromatography/mass spectrometry method to measure 3-BrY concentrations in serum from dogs has recently been established and analytically validated. The aims of this study were to determine the stability of 3-BrY in serum, to determine the association between peripheral eosinophil counts and the presence of an eosinophilic infiltrate in the gastrointestinal tract, and to compare serum 3-BrY concentrations in healthy dogs (n = 52) and dogs with eosinophilic gastroenteritis (EGE; n = 27), lymphocytic-plasmacytic enteritis (LPE; n = 25), exocrine pancreatic insufficiency (EPI; n = 26), or pancreatitis (n = 27).ResultsSerum 3-BrY concentrations were stable for up to 8, 30, and 180 days at 4°C, −20°C, and −80°C, respectively. There was no significant association between peripheral eosinophil count and the presence of eosinophils in the GI tissues (P = 0.1733). Serum 3-BrY concentrations were significantly higher in dogs with EGE (median [range] = 5.04 [≤0.63-26.26] μmol/L), LPE (median [range] = 3.60 [≤0.63-15.67] μmol/L), and pancreatitis (median [range] = 1.49 [≤0.63-4.46] μmol/L) than in healthy control dogs (median [range] = ≤0.63 [≤0.63-1.79] μmol/L; P < 0.0001), whereas concentrations in dogs with EPI (median [range] = 0.73 [≤0.63-4.59] μmol/L) were not different compared to healthy control dogs.ConclusionsThe present study revealed that 3-BrY concentrations were stable in serum when refrigerated and frozen. No relationship between peripheral eosinophil count and the presence of eosinophils infiltration in the GI tissues was found in this study. In addition, serum 3-BrY concentrations were increased in dogs with EGE, but also in dogs with LPE and pancreatitis. Further studies are needed to determine whether measurement of 3-BrY concentrations in serum may be useful to assess patients with suspected or confirmed EGE or LPE.
Highlights
Chronic enteropathy (CE) is characterized by recurrent or persistent gastrointestinal (GI) signs for more than 3 weeks [1,2]
There was no significant association between peripheral eosinophil count and the presence of eosinophils in the GI tissues (P = 0.1733; Figure 2)
No relationship between peripheral eosinophil count and the presence of eosinophils infiltration in the GI tissues was found in this study
Summary
Chronic enteropathy (CE) is characterized by recurrent or persistent gastrointestinal (GI) signs for more than 3 weeks [1,2]. Sattasathuchana et al BMC Veterinary Research (2015) 11:5 activity index (CIBDAI) can be used to evaluate the disease severity and treatment response in dogs with IBD [1,5]. No biomarker for eosinophilic infiltration has previously been identified in dogs with GI disease. The defining feature of EGE is infiltration of eosinophils in the GI tract. EGE can be caused by parasite infestation, neoplasia, allergy, IBD, or hypereosinophilic syndrome. Previous studies have reported that the histopathological findings of GI biopsies would suggest that EGE is secondary to LPE in canine IBD [6,7], eosinophils were shown to play crucial roles in stimulating inflammation and motility, leading to clinical signs such as diarrhea, inflammation, tissue destruction, fibrosis formation, and/or strictures [8,9]. A marker of eosinophil activation may provide a useful tool for evaluating the contribution of eosinophils in canine CE
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