Stability indicating square-wave stripping voltammetric method for determination of gatifloxacin in pharmaceutical formulation and human blood

Hanaa Salah El-Desoky

doi:10.1590/s0103-50532009001000004

Abstract

A fully validated, sensitive and precise stability-indicating square-wave adsorptive cathodic stripping voltammetric method has been developed for determination of gatifloxacin in the bulk form, pharmaceutical formulation, and in spiked human serum and real plasma samples. The achieved detection limits of gatifloxacin in the bulk form and human serum were 1.5 × 10-9 and 2.2 × 10-9 mol L-1, respectively. The described method was applied successfully for determination of gatifloxacin in formulation and human biological samples without extraction prior to the analysis. No significant interferences from common excipients, some common metal ions, organic species, co-administrated drugs and from the acid-induced degradation products were obtained during analysis of gatifloxacin in the various analyzed samples. Besides, pharmacokinetic parameters of gatifloxacin in plasma of healthy volunteers following the administration of an oral single dose (400 mg gatifloxacin) were also estimated by means of the described stripping voltammetric method.

Highlights

Gatifloxacin (1-cyclopropyl-6-fluoro-1, 4-dihydro-8methoxy-7-[3-methyl-1-piperazinyl]-4-oxo-3-quinoline carboxylic acid), (Scheme 1), is a synthetic broad-spectrum antimicrobial fluoroquinolone
Various instrumental analytical methods have been described for assay of gatifloxacin in bulk form, pharmaceutical formulation and biological fluids
Cyclic voltammograms of 1.0 ×10-4 mol L-1 gatifloxacin recorded at the hanging mercury drop electrode (HMDE) in the Britton-Robinson (B-R) universal buffer of pH values 3.5 to 8 exhibited a single 2-electron irreversible cathodic peak

Summary

Introduction

Gatifloxacin (1-cyclopropyl-6-fluoro-1, 4-dihydro-8methoxy-7-[3-methyl-1-piperazinyl]-4-oxo-3-quinoline carboxylic acid), (Scheme 1), is a synthetic broad-spectrum antimicrobial fluoroquinolone. Effect of accumulation potential (Eacc.) on the SW-AdCS voltammetric peak current magnitude of 5.0×10-7 mol L-1 gatifloxacin in a B-R universal buffer of pH 7 was examined over the range 0.0 to – 1.1 V (vs Ag/ AgCl/KCl ) following its preconcentration by adsorptive s accumulation onto the HMDE for 30 s.

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