Abstract
A Simplified Liquid Chromatography-Mass Spectrometry Method for Simultaneous Determination of Pyrimethamine, Sulphadoxine and Artesunate in Human PlasmaS M Sandhya, P S Shijikumar
Highlights
Malaria is an infectious disease commonly found in the tropical countries
The present work takes into account the development of Reverse Phase High Performance Liquid Chromatography (HPLC) for simultaneous method estimation and validation of pyrimethamine and sulfamethoxypyrazine in pharmaceutical formulation
This validated method can be suggested for the routine simultaneous laboratory analysis of pyrimethamine and sulfamethoxypyrazine
Summary
Malaria is an infectious disease commonly found in the tropical countries. Eukaryotic plasmodium parasites (mainly Plasmodium falciparum and Plasmodium vivax) are the root cause of this deadly disease. Pyrimethamine (PYR), an antimalarial drug, works by blocking the biosynthesis of pyrimidines and purines, which plays an important role in DNA synthesis and cell multiplication. This is achieved by inhibiting the dihydrofolatereductase of plasmodia. The study on fixed dose combination of artesunate/sulfamethoxypyrazine/pyrimethamine in treatment over 24 hours was reported (Penali and Jansen, 2008) For this combination, only a single method was reported using LC/MS in plasma samples (Storme et al, 2006). No study was performed on the selected combination of drugs by HPLC method, to determine the % assay in marketed formulation. Digital balance (Shimadzu) aided in weighing of the drugs for preparation of stock solutions
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