Abstract
Simple, sensitive and accurate thin layer procedure was described for a quantitative determination of paracetamol in its bulk powder and in its pharmaceutical dosage forms in the presence of its degradation product. The method consists of dissolving the drug in methanol and then spotting the solution on a thin layer of silica gel G254. Paracetamol was separated on silica gel using the mixture of the mobile phase, ethyl acetate: benzene: acetic acid in a ratio (1:1:0.05v/v/v).Absorbance measurements (detection of reflectance) of the separated drug were carried out at 250nm. Calibration curves were established in the concentration range of 5–20mcg/spot for paracetamol. Quantitation is achieved by comparing the area under the peaks obtained from scanning the thin layer chromatographic plates in a spectrodensitometer. The method has been successfully applied to pharmaceutical preparations (capsules) and the results obtained were statistically compared with those obtained by applying the reference method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
