Abstract
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Amlodipine and Losartan Potassium, in its pure form as well as in tablet dosage form. Chromatography was carried out on Acquity BEH-shield RP18 UPLC column (3.0 mm × 100) mm, particle size Column using a mixture of Acetonitrile and Acetate buffer (pH-4.3) (35:65% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 238nm. The retention time of the Amlodipine and Losartan Potassium was found to be 2.179, 3.610 ±0.02min respectively. The method produce linear responses in the concentration range of 20-60µg/ml of Amlodipine and 10-30µg/ml of Losartan Potassium respectively. The method precision for the determination of assay was below 2.0% RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
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