Stability control and its effect assessment of rat middle cerebral artery occlusion model

Abstract

Objective To search for the methods of inducing the high stability rat middle cerebral artery occlusion （MCAO） model and to noninvasively assess the method of model effect. Methods Six kinds of filaments with different diameters were used to induce rat MCAO models and their success rate, incidence of subarachnoid hemorrhage, and infarct volume were compared. The model scores were performed to assess the model effect after the operation and at 24 hours after reperfusion. Results Higher model success rate and lower incidence of subaracbnoid hemorrhage were achieved when the 0.28 mm in diameter and 0.32-0.34 mm in tip diameter filaments were used. The sensibility and specificity were higher when the model scores were performed at 24 hours after reperfusion. Conclusions Using the filaments of 0.28 mm in diameter and 0.32-0.34 mm in tip diameter for intraluminal thread could improved the stability of the models. The model scores at 24 hours after reperfusion could noninvasively assess the model effect. Key words: Arterial occlusive diseases; Cerebral arterial diseases; Brain infarction; Disease models, animal; Rats