Stability Constants for the Equilibrium Models of Iron(III) with Several Biological Buffers in Aqueous Solutions

Abstract

The complexation equilibria of Fe(III) with two buffer families, which are ubiquitous in biological system studies, were studied by potentiometric measurements at a constant ionic strength of I = 0.1 mol·dm−3 NaNO3 in aqueous solutions at 298.15 K. The members of TRIS family are tris(hydroxymethyl)aminomethane (TRIS), N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES), N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (TAPS), N-[tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid (TAPSO), and N-tris(hydroxymethyl)methyl-4-aminobutanesulfonic acid (TABS) buffers. The members of morpholine family are 4-morpholineethanesulfonic acid (MES), 4-morpholinepropanesulfonic acid (MOPS), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), and 4-(N-morpholino) butanesulfonic acid (MOBS) buffers. The overall stability constants were determined from pH-metric data using the least-squares curve-fitting program HYPERQUAD 2008. Based on the best-fit results, the species formed at equilibrium are ML, ML2, ML2H−1, and ML3 in the systems with TRIS family buffers. The complex species ML, ML2, ML2H−1, and MLH−1 are formed in the MOPSO-containing system, while ML, ML2, and ML2H−1 are formed in the systems with MES, MOPS, and MOBS. The stabilities of the complexes fall in the order TABS > TRIS > TAPS > TAPSO > TES and MOBS > MOPS > MOPSO > MES for the TRIS family and morpholine families, respectively.