Abstract
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.
Highlights
The epsilon toxin is produced by the Clostridium perfringens types B and D in a relatively inactive form called epsilon prototoxin (Rood and Cole, 1991; Payne and Oyston, 1997)
This study proposes an alternative method for the production of toxoids, free of contaminants, stable, and nontoxic, to be employed in the development of new vaccines for the prophylaxis of the fatal enterotoxemy, caused by the epsilon toxin of C. perfringens types B and D
The iodination of the prototoxin up to the saturation level resulted in a loss of its toxicity after activation with tripsin
Summary
The epsilon toxin is produced by the Clostridium perfringens types B and D in a relatively inactive form called epsilon prototoxin (Rood and Cole, 1991; Payne and Oyston, 1997). This study proposes an alternative method for the production of toxoids, free of contaminants, stable, and nontoxic, to be employed in the development of new vaccines for the prophylaxis of the fatal enterotoxemy, caused by the epsilon toxin of C. perfringens types B and D. The volume of KI3 added was recorded and used as the amount needed to achieve a 100% incorporation of iodine to a solution containing 2mg of the epsilon prototoxin.
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