Abstract

The storage stability of amperometric enzyme electrodes has been enhanced using a proprietary stabilising combination consisting of polycation D5106 and a sugar alcohol, lactitol. Different types of N -methylphenazinium tetracyanoquinonedimethane (NMP-TCNQ) mediated biosensors have been produced using the enzymes L-lactate oxidase, L-malate dehydrogenase and L-glutamate oxidase. The addition of the stabilising combination promoted an increase in the activity of the enzyme component during desiccation and conferred elevated storage stability on the enzyme, indicated by an increase in the shelf life of the desiccated biosensors under conditions of thermal stress at 37 °C. The results obtained from the biosensor activities on storage correlate approximately with the activity profiles observed for the enzymes in solution after desiccation in the presence of the stabilising combinations.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.