Abstract
The thiamine destruction by an extract of Laetiporus versisporus is not stimulated by pyridine but that by Boletus rubellus and Lactarius piperatus is remarkably stimulated. Thiamine-decomposed products by these mushrooms were studied by means of gaschromatography. The results obtained were summarized as follows. 1. 4-Methyl-5-β-hydroxyethyl thiazole (Th) was found mainly in the ether soluble part. 2. 2-Methyl-4-amino-5-hydroxymethyl pyrimidine (Pm) was found mainly in the butanol soluble part. 3. In the case of Lactarius piperatus, besides a big peak of Th, some small peaks of unknown substances were found, however, when pyridine was added, only one peak of pyrithiamine was observed. 4. In the case of Boletus rubellus, pyrithiamine did not appear when pyridine was added. Retention time of the main products was 3.2 and 11 minutes (pyrithiamine, 2.7). 5. In the case of Laetiporus versisporus, peaks of other unknown substances were much found than that of Th and Pm.
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