SSR markers associated to early leaf spot disease resistance through selective genotyping and single marker analysis in groundnut (Arachis hypogaea L.)

Adama Zongo,Pawan Khera,Mahamadou Sawadogo,Yaduru Shasidhar,Manda Sriswathi,Manish K Vishwakarma,Philippe Sankara,Bonny R Ntare,Rajeev K Varshney,Manish K Pandey,Haile Desmae

doi:10.1016/j.btre.2017.07.005

Adama Zongo, Pawan Khera + Show 9 more

Open Access

https://doi.org/10.1016/j.btre.2017.07.005

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Abstract

Groundnut (Arachis hypogaea L.) is an important oilseed and food crop of the world. Breeding for disease resistance is one of major objectives in groundnut breeding. Early leaf spot (ELS) is one of the major destructive diseases worldwide and in West Africa, particularly in Burkina Faso causing significant yield losses. Conventional breeding approaches have been employed to develop improved varieties resistant to ELS. Molecular dissection of resistance traits using QTL analysis can improve the efficiency of resistance breeding. In the present study, an ELS susceptible genotype QH243C and an ELS resistant genotype NAMA were crossed and the F2 population genotypic and F3 progenies phenotypic data were used for marker-trait association analysis. Parents were surveyed with 179 simple sequence repeat (SSR) markers out of which 103 SSR markers were found to be polymorphic between the parents. These polymorphic markers were utilized to genotype the F2 population followed by marker-trait analysis through single marker analysis (SMA) and selective genotyping of the population using 23 resistant and 23 susceptible genotypes. The SMA revealed 13 markers while the selective genotyping method identified 8 markers associated with ELS resistance. Four markers (GM1911, GM1883, GM1000 and Seq13E09) were found common between the two trait mapping methods. These four markers could be employed in genomics-assisted breeding for selection of ELS resistant genotypes in groundnut breeding.

Highlights

Groundnut or peanut (Arachis hypogaea L.), originated in South America, is one of the most important oilseeds and food crops cultivated in the semi-arid tropics
The results indicated that 13 markers were linked to Early leaf spot (ELS) disease resistance out of the 103 markers (Table 2)
The present study was undertaken to identify marker-trait association from a mapping population derived from resistant (NAMA) and susceptible (QH243C) parents for ELS

Summary

Introduction

Groundnut or peanut (Arachis hypogaea L.), originated in South America, is one of the most important oilseeds and food crops cultivated in the semi-arid tropics. The cultivated groundnut is tetraploid (2n = 4x = 40). It is member of genus Arachis and family Leguminosae [1]. The agro-morphological diversity within the crop, the differences in the branching pattern and presence of reproductive node on the main stem, allowed to distinguish the two cultivated subspecies i.e. A_hypogaea subsp. The subspecies are further divided into botanical varieties. The subspecies hypogaea is divided into hypogaea (virginia) and hirsuta, while the subspecies fastigiata into fastigiata (valencia), vulgaris (spanish), peruviana and aequatoriana [1]

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