Abstract
This study aims to determine the genetic structure of rainbow clam Moerella iridescens in different sea areas of China. Seventeen pairs of microsatellite primers (SSR) were used to amplify the SSRs of rainbow clam in Lianyungang of Haizhou Bay, Chongming of Shanghai, Ningde of Fujian, Daishan of Zhoushan, and Cixi and Wenzhou of Zhejiang. A total of 1,146 alleles were detected in 310 individuals from the 17 SSR loci. The average observed heterozygosity of six populations was 0.4381−0.6139, the average expected heterozygosity was 0.5897−0.7325, and the average Shannon diversity index was 1.2655−1.7998. The clams exhibited rich genetic diversity, and the FST of the genetic differentiation index of the six populations was 0.0470, indicating low genetic differentiation among the populations. The results indicated that rainbow clams along the coasts of China exhibited high diversity and low population differentiation.
Highlights
The rainbow clam Moerella iridescens is an economically important small-sized marine clam due to its delicious taste and high nutritional value
Pop32 software was utilized to calculate the number of effective alleles (Ne), expected heterozygosity (He), observed heterozygosity (Ho), and gene flow (Nm = 1− FST/4 FST, where the FST represent genetic differentiation index), genetic distance (Ds), and Shannon diversity index (Raymond and Rousset, 1995)
Except for the alleles detected at the two loci of HGZ2 and HGZ14, the remaining alleles were highly polymorphic loci, which indicated that the 17 microsatellite loci could be used to study the population genetics of rainbow clam (Table 3)
Summary
The rainbow clam Moerella iridescens is an economically important small-sized marine clam due to its delicious taste and high nutritional value. The clam is mainly distributed on the West Pacific coast and northern Australia. The rainbow clam has been increasingly used as high-valued seafood and is preferred by consumers of China, and the output of the rainbow. Genetic Resource Assessment of M. iridescens Stock LYG CM. Sampling time Number of samples Sampling site GPS. 2012·10 50 Sanyang harbor, Haizhou Bay E119.2835 N34.7996. 2012·10 50 East beach, Chongming Island E122.0058 N31.5316 ND. 2012·10 50 Xiabaishi, Sansha Bay in Fu’an E119.6263 N26.7948
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