SSR marker analysis and DNA fingerprinting of mutant rice variety VTL 10 (Lavanya)

Abstract

VTL-3 is a tall high yielding saline tolerant rice variety developed by Rice Research Station, Vyttila in 1987, which is having a tendency to lodge at maturity. Researchers hence, developed semi-tall lines of VTL-3 using induced mutagenesis. The selected semi-tall mutants were screened with markers linked to Saltol QTL and it was found that this region was not affected in the mutant. A promising line selected after comparative yield trials was later released as VTL 10, Lavanya in the year 2018. In order to differentiate the variety Lavanya from its parent VTL 3, SSR marker analysis and DNA fingerprinting was carried out. These two varieties were screened with 48 SSR markers for DNA fingerprinting and eight markers were found to be polymorphic between VTL-10 andVTL-3. The polymorphic markers include RM600, RM3362,RM279, RM7, RM85, RM149, RM144 and RM1880. The number of alleles ranged from 1 to 3 and the size of PCR products ranged from 110 to 300bp with Polymorphic Information Content (PIC) from 0 to 0.38. From the present study, it was observed that these eight markers can be used to distinguish the mutant line VTL-10 from its original parent VTL-3.