Abstract
The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.
 DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.
 According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers.
Highlights
Rosaceous genomes offer one of the best systems for the comparative study of genome evolution
In contrast to other members of Prunus, peach is a self-compatible species with a high level of inbreeding (Warburton and Bliss 1996)
Diversity of this crop has been drastically reduced by the use of improved varieties with a common genetic base from parents belonging to the same gene pool (Aranzana et al 2003, Bouhadida et al 2010)
Summary
Rosaceous genomes offer one of the best systems for the comparative study of genome evolution. Peach (Prunus persica (L) Batsch) is considered one of the genetically most well characterized species in the Rosaceae, and has been used as a model for genetic and genomic studies within its genus. According to Testolin et al (2000) several cultivars with great breeding value show a low degree of heterozygosity (Cipriani et al 1999, Dirlewanger et al 2002, Aranza et al 2003). Diversity of this crop has been drastically reduced by the use of improved varieties with a common genetic base from parents belonging to the same gene pool (Aranzana et al 2003, Bouhadida et al 2010)
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