SSCF-Hyperthermia Study in MCF-7 Spheroids – In Silicio

Abstract

Electromagnetic hyperthermia is an alternative treatment for cancer that has been carried out as an adjuvant to other cancer treatments such as chemotherapy and radiotherapy; this study shows the interaction between a cell culture of three dimensional MCF-7 spheroids (Michigan Cancer Foundation-7) inside a culture media and the electromagnetic radiation generated by a set of two applicator antennas. This study was performed by using an electromagnetic simulation model. The cell culture was modeled by using its conductivity and permittivity parameters and was placed in a 24-cell plate. The distribution of the electric field was analyzed in both, the culture media and the 3D spheroid culture MCF-7 when they were illuminated by the two applicators in different scenarios regarding the input power of the applicator and the distance between the cell culture and the applicator. Temperature behavior was analyzed from the electric field. Moreover, standardization of spheroids using microplates coated with Ultra-Low Attachment (ULA) surface for 3D cell culture (Corning® Costar®) through two methods, with and without the Geltrex™ LDEV matrixes is presented. The preliminary results indicate that the electric field is significantly more focused over the 3D spheroids in a scenario when the applicators are located 4 cm apart from the cell culture and are excited with a power of 5 W, in this scenario configuration the effect of hyperthermia will be successfully obtained for the 3D spheroids.