Abstract
BackgroundOur laboratory has shown that a locus on the SHR Y chromosome increases blood pressure (BP) in the SHR rat and in WKY rats that had the SHR Y chromosome locus crossed into their genome (SHR/y rat). A potential candidate for this Y chromosome hypertension locus is Sry, a gene that encodes a transcription factor that is responsible for testes development and the Sry protein may affect other target genes.MethodsThe following study examined if exogenous Sry would elevate adrenal Th, adrenal catecholamines, plasma catecholamines and blood pressure. We delivered 10 μg of either the expression construct, Sry1/pcDNA 3.1, or control vector into the adrenal medulla of WKY rats by electroporation. Blood pressure was measured by the tail cuff technique and Th and catecholamines by HPLC with electrochemical detection.ResultsIn the animals receiving Sry there were significant increases after 3 weeks in resting plasma NE (57%) and adrenal Th content (49%) compared to vector controls. BP was 30 mmHg higher in Sry injected animals (160 mmHg, p < .05) compared to vector controls (130 mmHg) after 2–3 weeks. Histological analysis showed that the electroporation procedure did not produce morphological damage.ConclusionThese results provide continued support that Sry is a candidate gene for hypertension. Also, these results are consistent with a role for Sry in increasing BP by directly or indirectly activating sympathetic nervous system activity.
Highlights
Our laboratory has shown that a locus on the SHR Y chromosome increases blood pressure (BP) in the SHR rat and in WKY rats that had the SHR Y chromosome locus crossed into their genome (SHR/y rat)
The plasma NE values of the two electroporated groups was similar to previous values in WKY reported from our lab suggesting that the electroporation procedure did not damage the adrenal medulla[20]
Typical effects on BP attributed to other candidate genes or loci are small or equal to the 30 mmHg effect we find after SHR Sry delivery to WKY adrenal gland
Summary
Our laboratory has shown that a locus on the SHR Y chromosome increases blood pressure (BP) in the SHR rat and in WKY rats that had the SHR Y chromosome locus crossed into their genome (SHR/y rat). We have shown previously that there is a locus on the SHR Y chromosome that increases blood pressure (BP) about 20–25 mmHg [1,2]. Further studies showed that the SHR Y chromosome increased several indices of SNS activity [4]. Our recent studies indicate that a candidate gene for this SNS and BP effect is Sry, a transcription factor on the Y chromosome that is the testis determining factor [6]. Sry expression has been reported in testis, the brain and in (page number not for citation purposes)
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