SRGN enhances the breast cancer cells metastasis by inducing Epithelial-mesenchymal transition

Abstract

Objective To investigate the mechanism of glycoprotein serglycin (SRGN) promoting metastasis of breast cancer cells and the possible mechanism of SRGN expression. Methods Real time quantitative polymerase chain reaction (PCR) and bioinformation retrieval were used to detect the expression of SRGN in lymph node metastasis and non-metastasis breast cancer. MDA-MB-231shRNA and MCF-7-SRGN of breast cancer stable cell line were established by lentivirus shRNA interference and overexpression. Transwell assay was used to test the effect of SRGN on invasion and metastasis of breast cancer cell line in vitro. Western blot assay was used to detect the changes of epithelial-mesenchymal (EMT) related markers. The possible regulatory mechanism of SRGN expression was detected by Western blot assay. Results SRGN expression was significantly increased in lymph node metastasis of breast cancer in clinical specimens. SRGN interference inhibited the invasion and metastasis of tumor cells. SRGN promoted breast cancer cells EMT. Transforming growth factor β1 (TGFβ1) promoted the expression of beta SRGN transcription. Conclusions SRGN can induce the change of EMT in breast cancer cells and promote the invasion and metastasis of breast cancer cells. Key words: Proteoglycans; Breast neoplasms; Tumor cells, cultured; Biotransformation; Neoplasm invasiveness; Neoplasm metastasis