Square salads: exploring the diet of the southern hairy-nosed wombat (Lasiorhinus latifrons) via DNA metabarcoding

Abstract

Southern hairy-nosed wombats (Lasiorhinus latifrons) are large semi-fossorial marsupials that inhabit semiarid areas of southern South Australia and southeast Western Australia. Despite their size and prominence on the landscape, little is published about diet and foraging preferences. Further, it is unclear whether this wombat species’ diet overlaps with sympatric western grey kangaroos (Macropus fuliginosus), which are locally abundant across the southern hairy-nosed wombat’s distribution. Competition for food resources may be an added stressor in Murraylands populations where kangaroos are plentiful. Here, we identified diet items and dietary overlap of southern hairy-nosed wombats and western grey kangaroos. We accomplished this by opportunistically sampling fresh faecal pellets from both species at Brookfield Conservation Park in April 2017. We performed DNA metabarcoding via Illumina next generation sequencing of the faecal DNA using the plant ITS2 gene. We identified 10 genera in the wombat diet and 20 in the diet of kangaroos; diets of both species included native and introduced genera. Eight genera were shared between both marsupials. This work highlights the capabilities of non-invasive genetic sampling in concert with DNA metabarcoding to elucidate diet and dietary overlap between species.