Abstract
The in vitro block in cholesterol biosynthesis which causes squalene accumulation in brain preparations has been investigated using 3 H-squalene and assaying for labeled squalene-2,3-oxide formation. It has been determined that: 1) squalene epoxidase activities in brain and liver are comparable; 2) there is no change in brain epoxidase activity corresponding to the peak in brain cholesterol synthesis during myelination; 3) the epoxidase is inhibited 3.5-fold by a squalene-2,3-oxide trap. These facts indicate that brain epoxidase activity is not a control point for brain cholesterol biosynthesis during development, in spite of the marked squalene accumulation observed in vitro .
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