SPR-HPV detection of human cervical papillomavirus infection in clinical research

Abstract

Objective To study the status of surface plasmon resonance (SPR) detection of cervical human papillomavirus (HPV) infection in clinical practice.Methods 502 cases of cervical HPV in patients with cervical cell's genetic subtypes were diagnosed using SPR.Positive rates of HPV lesions of cervical levels detected by SPR and nucleic acid molecules rapid flow-through hybridization gene chip (HybriMax) technology were compared.Results Twenty-two kinds of subtypes were detected by SPR.106 women were found with single infection of HPV,including 47 women (44.34％) with abnormal cytological results,while 36 women were found with multiple infection of HPV,including 16 women (44.44％) with abnormal cytological results,and the difference was not significant (P＞0.05).Among 106 women with single infection of HPV,93 women were found with high risk HPV infection,including 45 women (48.39％) with abnormal cytological results,while 13 women were found with middle-low risk HPV infection,including 2 women (15.38％) with abnormal cytological results,and the difference was statistically significant (P＜0.005).Comparison of SPR and HybriMax technology showed that the two test results showed no significant difference (P＞0.05).Conclusions SPR for detection of HPV infection is more reliable,it can combine HybriMax as the primary means of clinical detection. Key words: Surface plasmon resonance technology; Human papillomavirus; Clinical research