Spread of nanostructure of S-Layer among the bacteria isolated from Skin

Abstract

An S-layer (surface layer) is a part of the cell envelope commonly found in bacteria, as well as among archaea. It consists of a monomolecular layer composed of identical proteins or glycoproteins. As for many bacteria, the S-layer represents the outermost interaction zone with their respective environment, its functions are very diverse and vary from species to species; subject of this study was to find out the prevalence of Nanostructure of Surface layer among the Bacillus cereus isolated from the skin of clinical staff. Relevant information to Surface Layer was extracted from articles that listed in Pubmed, Elsivier Science, and Yahoo from 1995 to 2010 years. The research was performed with laboratory method during 2005-2007 years in Azzahra Hospital and Esfahan University. Bacterial identification was performed with standard bacteriological methods and selective culture medium was applied in preparation of samples. Bacterial samples were cultured in TSA, for 16 hour, in aerobic condition then separated the surface proteins to run electrophoresis with molecular weight marker. S-Layer in Bacillus cereus has 97KD molecular weight. Among the 13 Bacillus cereus strains isolated from skin of staff, 11 (84.60%) showed positive for S-layer. S-layers in pathogenic bacteria offer protection against bacteriophages and phagocytosis, resistance against low pH ,adhesion, stabilisation of the membrane and provide adhesion sites for exoproteins due to more pathogenesis, infection resistant and antibiotic resistant to bacteria. Result of this study shows the prevalence of S-layer in pathogen bacteria and as important to determine S-layer producer strains in laboratory.