Abstract
Inoculation of wheat seedlings by the crown rot pathogen Fusarium pseudograminearum is commonly used to screen for resistance based on leaf sheath discolouration. While leaf sheath discolouration is effective for describing disease reactions, the degree to which the pathogen colonises the full range of seedling host tissues is an important aspect of the disease process, which has not been systematically examined. After single point droplet inoculation of the coleoptile, quantitative PCR was used to determine the spread of F. pseudograminearum into different host tissues during crown rot pathogenesis at 14 and 28 days after inoculation. Quantitative PCR and visual assessment indicated that while most seedling tissues (leaf sheath, leaf blade, sub-crown internode, primary root and secondary root) were colonised, the basal portion of leaf sheath tissue supported the highest density of F.pseudograminearum. Other significant sites of colonisation were the upper leaf sheath and sub-crown internode. By comparison, the quantity of mycelium detected in the primary and secondary roots was significantly lower, while the infection of leaf blade tissues was severely restricted.
Published Version
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