Spraying silicon to decrease inorganic arsenic accumulation in rice grain from arsenic-contaminated paddy soil

Abstract

Addition of Si to soil can reduce As uptake and accumulation in rice, while also enhancing As release from soil particles via competing sorption sites in soil minerals with As. Foliar application of Si might be an alternative pathway to reduce As accumulation in rice. It is not clear which growing stage would be better for spraying different types of Si solution to reduce inorganic As in edible parts of rice. Soil pot experiments were conducted to investigate whether total As and inorganic As accumulation in rice grains was alleviated via spraying Si in As-contaminated paddy soil. The results showed that foliar Si application at the tillering or jointing stage significantly reduced As concentrations in rice husks and grain via inhibiting the translocation of As from straw to husk or grain compared with other growing stages. Spraying of Si at the tillering stage markedly decreased the concentrations of inorganic As in rice bran and polished rice, accounting for 27.3% and 61.4% respectively. Furthermore, spraying Si solution mixed with surfactant-Tween 80 not only dramatically reduced the total As in rice tissues by 48.8%, but also significantly alleviated the accumulation of inorganic As in rice grain by 49.2%. Spraying Si restricted inorganic As in rice grain through the mechanism by which foliar Si application at tillering stage increased the Si concentrations in shoot and root, which downregulated Si transporters of Lsi1 and Lsi2 in the root and Lsi6 in the blade and sheath significantly, and finally decreased As uptake and transport. Therefore, spraying Si is an alternative and efficient pathway to reduce inorganic As accumulation of rice grain in As-contaminated soil.