Spray-deposited Ag nanoparticles on micro/nano structured Ti6Al4V surface for enhanced bactericidal property and cytocompatibility

Abstract

In this study, different surface textures (Diamond, Cylinder, and Sin) have been designed and produced on Ti6Al4V alloys by selective laser melting technology. TiO 2 /Ag composite coatings were prepared on these textures by merging anodic oxidation and spray deposition methods. Tightly arranged TiO 2 nanotubes (TNTs) with tube diameters of about 100 nm are found on the top and lateral sides of the textures. On the bottom of the textures, dense nanopores were found with a pore diameter of about 50 nm. Compared with the untreated titanium alloy surface, both texture and TNTs structures would endow the titanium alloy surfaces with higher in vitro bioactivity. All three textures have an enhanced antibacterial property and a complete bactericidal effect was achieved by spray deposition of Ag nanoparticles (AgNPs) on all the surfaces. The MC3T3-E1 cells can adhere to the surfaces and proliferate normally, indicating good cytocompatibility of TiO 2 /Ag surfaces. Besides, the deposited AgNPs have been found to promote cell proliferation . • Tightly TiO 2 nanotubes have been prepared on surface-textured Ti6Al4V surfaces. • Ag Nanoparticles were spray-deposited onto micro/nano structures. • Both texture and TNTs structures would endow the titanium alloy surfaces with higher in vitro bioactivity and antibacterial property. • The deposited AgNPs have been found to promote cell proliferation.