Abstract
Amperometric miniature glucose and lactate sensors were integrated into the SPR immunosensor chip fabricated by the thin-film technology and the integrated sensor for simultaneous measurement of human immunoglobulin G (IgG), glucose and lactate were developed. The following problems caused by the integration of immuno- and enzyme sensors were discussed: (1) increasing the dynamic range of enzyme sensors; (2) decreasing the measurement time of the immunosensor; and (3) Effect of regeneration buffer on the enzyme sensor activities. Finally the integrated sensor was applied to the model experiment of on-line monitoring of animal cell culture bioreactor.
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