SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material.

A. Sankiewicz,E. Gorodkiewicz,B. Cylwik,B. Zelazowska-Rutkowska,P. Laudanski,L. Romanowicz,B. Puzan

doi:10.1007/s00216-016-9621-x

A. Sankiewicz, E. Gorodkiewicz + Show 5 more

Open Access

https://doi.org/10.1007/s00216-016-9621-x

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Abstract

A new method for the selective determination of laminin-5 concentration using a biosensor and surface plasmon resonance imaging (SPRI) technique is presented. A biosensor based on the specific interaction of laminin-5 with rabbit polyclonal antibody was constructed. The analytically useful dynamic response range of the biosensor is between 0.014 and 0.1 ng mL−1. The detection limit is 4 pg mL−1. The potential influence of interferences on the SPRI signal was investigated, and the high selectivity of the biosensor was confirmed. In order to demonstrate the potential application of the biosensor, laminin-5 concentration in blood plasma was determined. The results were compared with the laminin-5 concentration obtained by the commercial enzyme-linked immunosorbent assay (ELISA) kit. A comparison of results from healthy donors obtained by SPRI measurement and ELISA indicates that they are close and shows good agreement with the data reported in the literature. The plasma samples of bladder cancer patients gave higher concentration measured with specific biosensor than by ELISA assay. The study shows the clear difference in concentration of laminin-5 in healthy humans and patients with bladder cancer. Extensive clinical studies using the newly developed method can result in an increase in the use of laminin-5 as a potential cancer marker.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-9621-x) contains supplementary material, which is available to authorized users.

Highlights

Surface plasmon resonance imaging (SPRI) is a tool to monitor biomolecular interactions
The combination of SPRI technique with the biosensors is at the forefront of analytical methods of multi-analyte measurements [1]
Surface plasmon resonance (SPR) is generated at a thin metal surface when surface plasmons are excited by the light beam at an appropriate incident angle

Summary

Introduction

Surface plasmon resonance imaging (SPRI) is a tool to monitor biomolecular interactions. The combination of SPRI technique with the biosensors is at the forefront of analytical methods of multi-analyte measurements [1] This method measures the changes of refractive index caused by molecules bound to the metal surface. Surface plasmon resonance (SPR) is generated at a thin metal surface when surface plasmons are excited by the light beam at an appropriate incident angle. This angle strongly depends on the refractive index of the dielectric material at the interface. The biosensor is usually gold-coated glass with a layer of active biomolecule as a receptor for a determined compound They are based on very specific interactions between enzyme and inhibitor or between antigen and antibody. SPR imaging biosensors have been increasingly applied in medical diagnosis in which rapid and sensitive methods are required for the determination of substances (presence and/or amount) that are potential markers of disease and especially cancer markers [4,5,6,7,8]

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