SPR Biosensor Sensor principle and application progress

Abstract

SPR Biosensor, full name Surface Plasmon Resonance Biosensor, is an instrument used to detect biomolecular interactions. It has the advantages of high sensitivity, high selectivity and real-time to monitor the interaction between substances in real time by optical principle. The working principle of SPR biosensor is based on surface plasmon resonance effect. When there is a certain refractive index difference between the metal surface and the medium, the incident light will be reflected and transmitted. When a biological molecule or chemical molecule binds to a metal surface, it changes the refractive index of the surface, which causes the Angle and intensity of the reflected light to change. By monitoring the changes in reflected light, the binding process and properties of substances can be obtained. SPR biosensors can be widely used in biomedical fields, such as drug screening, disease diagnosis, protein interaction research and so on. Its simple principle, high sensitivity and strong real-time performance make it one of the indispensable tools in modern biological science research. The development direction of SPR biosensor mainly includes improving sensitivity and selectivity, expanding application field, reducing cost and so on. At present, many improved technologies and methods have emerged, such as the introduction of nanomaterials, microfluidic technology, etc., to improve the performance and practicality of sensors. In conclusion, SPR biosensor is an important bioanalytical instrument with broad application prospects and plays an important role in biomedical research and clinical diagnosis.