Abstract
A qualitative iodometric test for lipoxygenase activity is described. The sensitive method is useful for rapidly screening large numbers of samples, as from a chromatography column or after enzyme inactivation treatment in food processing.
Highlights
A TEST FOR LIPOXYGENASE is described which is adopted from a procedure for the detection of lipoxygenase bands in polyacrylamide gels (1)
The substrate, sodium linoleate, is prepared as for that used in the spectrophotometric method (3), except that sodium phosphate buffer is used. 1 ml (1.11 g) of Tween 20 is dissolved in 20 ml of 0.046 M sodium phosphate buffer. 1 g of linoleic acid is added dropwise with stirring, 4 ml of N NaOH is added dropwise and mixed until a clear solution is obtained
When using substrate which has been stored longer than a few days, control tests containing no active lipoxygenase must be made simultaneously with sample tests, because traces of peroxides from the stored substrate cause oxidation of iodide, which begins to be visible in the absence of enzyme about 10 min after the addition of aqueous KI to a reaction mixture
Summary
WALLACE Western Regional Research Laboratory, Agricultural Research Service, U.S Department of Agriculture, Berkeley, California 94710. Summary A qualitative iodometric test for lipoxygenase activity is described. The sensitive method is useful for rapidly screening large numbers of samples, as from a chromatography column or after enzyme inactivation treatment in food processing
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