SPOT Synthesis of Peptide Arrays on Self‐Assembled Monolayers and their Evaluation as Enzyme Substrates

Abstract

amino acid to the other, and therefore the amount and purity of peptides can also vary. Monitoring reactions on the support relies on the use of a coloured dye, and quality control of the peptide requires its cleavage from the support after completion of synthesis for subsequent solution-phase analysis by HPLC or MALDI-ToFMS. [19] Consequently, assay results obtained from an array prepared by SPOT synthesis sometimes need to be confirmed by using purified samples of peptides in a solution-phase assay. [15] By combining the robustness and versatility of SAM-coated gold platforms and the easy-to-handle SPOT peptide synthesis methodology, an efficient method for preparing peptide arrays on gold surfaces is described herein. The method allows onchip monitoring of the synthesis and subsequent evaluation of enzymatic modifications by MALDI-ToFmass spectrometry (Figure 1).