Sporulation of Bacillus popilliae in liquid medium as affected by kind of carbon and method of sterilization.

Abstract

Of six activated carbons from two manufacturers, four made from nutshells evoked sporulation by Bacillus popilliae NRRL B-2309S in a tryptone–glucose–yeast extract – phosphate medium. Four to nine million refractile spores per milliliter were produced in cultures containing a selected carbon that was sterilized apart from the base medium. A reduction in yield of spores that resulted from sterilizing glucose in the medium was moderated when carbon was also present during sterilization.