Abstract
Of six activated carbons from two manufacturers, four made from nutshells evoked sporulation by Bacillus popilliae NRRL B-2309S in a tryptone–glucose–yeast extract – phosphate medium. Four to nine million refractile spores per milliliter were produced in cultures containing a selected carbon that was sterilized apart from the base medium. A reduction in yield of spores that resulted from sterilizing glucose in the medium was moderated when carbon was also present during sterilization.
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