Abstract
The production of maximum numbers of Bacillus stearothermophilus spores was investigated by modifications of a liquid medium and environmental factors. Supplementation of trypticase soy broth with CaCl 2 (25 ppm), FeSO 4 . 7H 2 O (31 ppm), MnSO 4 . H 2 O (30 ppm) and MgCl 2 . 6H 2 O (11 ppm), resulted in 75% (5.0 × 10 8 phase bright spores ml −1 ) sporulation. The D 250 °F Value of the resulting spores was 2.9 min in distilled water, and 7.8 min in 1.0 M NaCl solution. D values of the spores suspended in sterilized milk at 115.5 °C (240 °F), 121.1 °C (250 °F) and 126.6 °C (260 °F) were found to be 22.4, 3.5 and 0.37 min, respectively.
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