Abstract
The sporulation abillity, varied among isolates, was easily lost when cultures were transferred with mycelial or conidial mass, but could be maintained by single-conidium culture method. This ability could be recovered when the culture was subcultured by single hyphal-tip method. Temperature and media, i.e., PDA, carrot agar and oatmeal agar, could affect both sporulation and germination of conidia. Whether or not the sporulation ability was influenced by sequential mycelial mass transfer depended on the isolates used and on the intervals when transferred. When conidia germinated, four phenomena could be observed, (i) emergence of germ tube, (ii) formation of dark-colored appressoria, (iii) anastomosis between two conidia or germ tubes, and (iv) production of secondary conidia.
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