Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea.

Abstract

A Gram-stain positive, non-motile, rod-shaped bacterium, designated strain 1111S-42(T), was isolated from the East Siberian Sea. The organism was found to grow at 4-30 °C, pH 7.0-8.5 and in 0-8 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 7.5 and in 1 % NaCl. Based on 16S rRNA gene sequence similarity studies, strain 1111S-42(T) was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915(T) (97.3 %) and Sporosarcina soli I80(T) (97.2 %). The main polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was identified as MK-7. The major cellular fatty acids were identified as anteiso-C15:0 (34.4 %), iso-C15:0 (29.8 %) and anteiso-C17:0 (22.4 %). The DNA G+C content of strain 1111S-42(T) was determined to be 39 mol %. The values of DNA-DNA relatedness between the strain 1111S-42(T) and related type strains of the genus Sporosarcina were less than 30 %. Based on the phylogenetic analysis, along with extensive physiological and chemotaxonomic testing, we conclude that the bacterium represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siberiensis sp. nov. is proposed. The type strain is strain 1111S-42(T) (=CGMCC 1.12516(T) = LMG 27494(T)).