Abstract
Spore production ofRamularia areolahas always been a difficult task. Brazilian isolates ofR. areolaproduce spores of variable size and shape. The typical spores are 3 septate, rarely 4 and 5 septate, together with abundant single celled oblong to round bodies—a phenomenon not reported earlier forR. areola. Budding of spores is a continuous process as observed in our isolates. By repeated culturing the pathogen either ceases to produce typical spores or loses its pathogenic character. To solve this problem, a technique was developed to produce large quantity of typical spores under laboratory conditions. Sporulating cultures produced on Petri plates containing V8 juice-agar were kept on the laboratory bench till they became dry and then stored at 5°C for reisolation and/or for production of fresh inoculum. In such dried cultures spores remain viable for a period of over 12 months, and hence isolates ofR. areolaoriginatedfrom different geographic regions can be maintained in sporulating form. Results of the present investigation would aid cotton breeders and pathologists in screening germplasm resistant toRamulariaand in other genetical studies under glasshouse conditions.
Highlights
Leaf blight of cotton (Gossypium hirsutum, G. arborium, G. herbaceum and G. barbadense) caused by Ramula-How to cite this paper: Volponi, J., Matos, J.N., Girotto, L., Marangoni, M.S., Galbieri, R. and Mehta, Y.R. (2014) Spore Types and Spore Production of Ramularia areola for Screening Cotton Germplasm for Resistance
A few years ago Ramularia leaf blight was of secondary importance in Brazil, since it used to occur almost at the end of the crop cycle
In recent years the disease has gained top-most importance in major cotton growing States of Brazil, such as São Paulo, Mato Grosso, Mato Grosso do Sul, Minas Gerais and Bahia
Summary
Leaf blight of cotton (Gossypium hirsutum, G. arborium, G. herbaceum and G. barbadense) caused by Ramula-How to cite this paper: Volponi, J., Matos, J.N., Girotto, L., Marangoni, M.S., Galbieri, R. and Mehta, Y.R. (2014) Spore Types and Spore Production of Ramularia areola for Screening Cotton Germplasm for Resistance. A few years ago Ramularia leaf blight was of secondary importance in Brazil, since it used to occur almost at the end of the crop cycle. In recent years the disease has gained top-most importance in major cotton growing States of Brazil, such as São Paulo, Mato Grosso, Mato Grosso do Sul, Minas Gerais and Bahia. In these States, at present the disease is being partially controlled through 6 - 8 aerial applications of systemic fungicides during the crop cycle. Considering the high cost of fungicidal applications allied with the problem of creation of mutants resistant to some fungicides, efforts are being made to control the disease through varietal resistance
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