Spore Germinability of Bacillus coagulans and Acidophile Bacillus spp. Isolated from PET-bottled Tea and Juices.

Abstract

We examined the optimal conditions for germination of the spores of Bacillus coagulans and an acidophile Bacillus spp isolated from PET-bottled drinks on the market containing Japanese tea or orange or apple juice. Among the spores of B. coagulans BUB strains, isolated from Japanese tea, BUB46 germinated effectively at an acidic pH in a mixture of D-glucose plus KNO3 (G+K), and in a mixture of L-asparagine, D-glucose, D-fructose and KCI (AGFK) at 45 or 53°C, but not by L-alanine. The spores of BUB130 germinated in a mixture containing G+K, AGFK or L-alanine, and BUB44 spores also germinated most effectively in an acidic solution of AGFK. Pretreatment of the BUB44 spores with 1/16N NaOH stimulated germination, but that with 1% sodium dodecyl sulfate plus 5 mM dithiothreitol did not. The spores of an acidophile Bacillus spp. isolated from fruit juice germinated at pH 3.0 in a mixture containing G + K, AGFK, L-alanine or potato dextrose.