Abstract
We have determined the frequency of rosette-forming cells (RFC) in lymphocytes from the efferent lymph of sheep. Very low numbers of RFC were present in the efferent lymph of non-immunized lymph nodes, but after immunization with heterologous erythrocytes the frequencies of specific RFC and of plaque-forming cells (PFC) increased. The number of RFC remained elevated following the decline and ultimate disappearance of PFC in the lymph. Although most PFC were found to be RFC, the converse was not true. The number of RFC in efferent lymph always exceeded the number of PFC. Most RFC were found in nylon wool-adherent purified B cell fractions, whereas the highest number of PFC were regularly found in the cell fraction which passed through the nylon wool.
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