Spontaneous Repolarization Alternans Causes VT/VF Rearrest That Is Suppressed by Preserving Gap Junctions.

Abstract

Ventricular tachycardia (VT)/ventricular fibrillation (VF) rearrest after successful resuscitation is common, and survival is poor. A mechanism of VT/VF, as demonstrated in exvivo studies, is when repolarization alternans becomes spatially discordant (DIS ALT), which can be enhanced by impaired gap junctions (GJs). However, invivo spontaneous DIS ALT-induced VT/VF has never been demonstrated, and the effects of GJ on DIS ALT and VT/VF rearrest are unknown. This study aimed to determine whether spontaneous VT/VF rearrest induced by DIS ALT occurs invivo, and if it can be suppressed by preserving Cx43-mediated GJ coupling and/or connectivity. We used an invivo porcine model of resuscitation from ischemia-induced cardiac arrest combined with exvivo optical mapping in porcine left ventricular wedge preparations. Invivo, DIS ALT frequently preceded VT/VF and paralleled its incidence at normal (37°C, n=9) and mild hypothermia (33°C, n=8) temperatures. Maintaining GJs invivo with rotigaptide (n=10) reduced DIS ALT and VT/VF incidence, especially during mild hypothermia, by 90% and 60%, respectively (P< 0.001; P< 0.013). Exvivo, both rotigaptide (n=5) and αCT11 (n=7), a Cx43 mimetic peptide that promotes GJ connectivity, significantly reduced DIS ALT by 60% and 100%, respectively (P< 0.05; P< 0.005), and this reduction was associated with reduced intrinsic heterogeneities of action potential duration rather than changes in conduction velocity restitution. These results provide the strongest invivo evidence to date suggesting a causal relationship betweenspontaneous DIS ALT and VT/VF in a clinically realistic scenario. Furthermore, our results suggest that preserving GJs during resuscitation can suppress VT/VF rearrest.