Abstract
A low-density subpopulation of normal human peripheral blood mononuclear leukocytes obtained by percoll gradients was found to produce significant levels (up to 10,000 units/ml) of interferon in the absence of an external inducer. The interferon (IFN) was characterized as a mixture of IFN-γ (immune) and a pH-sensitive IFN-α by neutralization with specific antibodies, by lability to low pH, and by cross-reactivity on bovine cells. Significant levels of IFN were obtained only at high (⩾5 × 10 6 cells/ml) cell densities. These levels were not affected by the presence of small T lymphocytes and sharply decreased with cell dilution in a nonlinear fashion. A release of IFN in the absence of an external inducer, indicates additional physiological roles besides its involvement in pathological conditions.
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