Abstract
Abstract— Oxidation of β-dehydro nicotineamide adenine dinucleotide (NADH) by oxygen catalyzed by horseradish peroxidase was accompanied by spontaneous, low-level chemiluminescence in the existence of co-oxidant 2,4-dichlorophenol. Electrochemical oxidation of NADH by the glassy carbon electrode also emitted a spontaneous low-level chemiluminescence above the potental +0.7 V vs reference electrode (Ag/AgCl) in aqueous medium in pH 7.0˜10.0. Low-level electrochemilumin-escence was observed when the NAD and oxygen species coexisted in the neighborhood of the electrode surface. The main photon emission process was supposed to be generated by the interaction of pyridine moiety of NAD and oxygen.
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