Spontaneous cyclic contractions of the capillary wall in vivo, impeding red cell flow: A quantitative analysis : Evidence for endothelial contractility

Abstract

We have videorecorded spontaneous cyclic contractions of capillary walls which often stopped the flow of blood cells, in spleens of rat and mouse. An inverted microscope and oblique lighting were key elements in obtaining images in which the boundaries of cells composing vessel walls were clearly distinguishable. Using slow motion replay, we measured the widths of endothelial cells ( C), pericytes ( P, when present) and capillary lumens ( L; at the site of constriction; U; 15–20 μm upstream), throughout 11–12-min sequences containing many contraction/relaxation cycles. In roughly 50% of contractions L decreased to 0–1 μm, such luminal “closures” occurring within 2–12 sec and lasting for < 1 sec to > 1 min. Intervals between contractions ranged from 12 sec to 3 min (average 1 min). Documentation of one such cycle by sequential photographs from the video monitor is presented, showing dramatic bulging of an endothelial cell into the lumen. Comparison of records of L and C versus time showed that almost invariably when L decreased C increased, and vice versa; highly significant correlations existed between C and L in every case ( P < 0.0005). Multiple linear regression analysis showed that changes in C were responsible for 18–77% of the total variance in L, whereas P contributed only 0–4%; changes in U, covariance between C, P, and U, and unexplained variance were responsible for 0–20, 11–30, and 11–53%, respectively, of the total variance in L. We conclude that these spontaneous capillary contractions were primarily due to endothelial contractility.