Spontaneous and lipopolysaccharide-stimulated secretion of cytokines by peripheral blood monocytes in IgA nephropathy is inhibited by interleukin-10.

Abstract

The effect of recombinant human interleukin-10 (IL-10) on the in vitro cytokine secretion of peripheral blood monocytes (PBM) was evaluated in patients with IgA nephropathy (IgAN). Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated secretion of tumor necrosis factor-alpha and interleukin-8 was determined in PBM cultures of IgAN patients compared to those of normal controls. In the present study IL-10 inhibited the spontaneous as well as the LPS-stimulated cytokine secretion of PBM in IgAN. A significant inhibitory effect of IL-10 was observed in cultures of PBM from IgAN patients as well as from normal controls. When both LPS and anti-IL-10 antibody were added together to the PBM, a further increase in LPS-enhanced secretion of cytokines occurred. This study provides some novel information showing the inhibitory effects of IL-10 on cytokine secretion by PBM derived from IgAN patients.