Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing.

Yu Cheng,Zuhao Zou,Sidong Li,Bijun Zhang,Sitong Lu,Zhang Hu,Yuntao Zhao

doi:10.3390/ijms20163890

Yu Cheng, Zuhao Zou + Show 5 more

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https://doi.org/10.3390/ijms20163890

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Abstract

Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC–COP) were prepared by covalent coupling and freeze drying. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were then used to characterize the prepared sponges. To evaluate the wound healing activity of the CMC–COP sponges, in vitro tests including cell viability scratch wound healing and scald wound healing experiments were performed in rabbits. Appearance studies revealed the porous nature of sponges and FTIR spectroscopy demonstrated the successful incorporation of COP into CMC. The in vitro scratch assay showed that treatment with CMC–COP sponges (at 100 μg/mL) had significant effects on scratch closure. For burn wounds treated with CMC–COP, regeneration of the epidermis and collagen fiber deposition was observed on day 7, with complete healing of the epidermis and wound on days 14 and 21, respectively. Based on the pathological examination by hematoxylin and eosinstaining, the CMC–COP group demonstrated pronounced wound healing efficiencies. These results confirmed that the CMC–COP treatment enhanced cell migration and promoted skin regeneration, thereby highlighting the potential application of these sponges in burn care.

Highlights

Burns are one of the most common injuries in daily life
The occurrence of superinfection is a major problem in the management of burns, causing secondary physical and psychological damage to patients
After magnetic stirring at the same temperature for a period of time, the reaction mixture was dialyzed for 3 d and freeze dried to obtain the carboxymethyl chitosan (CMC)–collagen peptides (COP) sponges (Freeze dryer: FD-1A50, Bilon, Shanghai, China)

Summary

Introduction

The occurrence of superinfection is a major problem in the management of burns, causing secondary physical and psychological damage to patients. Carboxymethyl chitosan is prepared by the introduction of a carboxymethyl group from chloroacetic acid to the -OH and -NH2 groups in chitosan This results in an increase in the water solubility and pH sensitivity of carboxymethyl chitosan, making it a widely used chitosan derivative in the biomedical field [2]. It is highly soluble in neutral and alkaline solutions and exhibits better sensitivity, biocompatibility, biodegradability, and moisture-retaining capacity than chitosan alone [3,4]. Carboxymethyl chitosan can reportedly inhibit the formation of algogenic substances, such as histamine, serotonin, and bradykinin, as well as prevent scar formation and promote normal fibrocyte growth [7,8]

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