Abstract
Laboratory selection of a strain of Spodoptera littoralis Boisduval with spore-crystal preparations of the Bacillus thuringiensis CryIC toxin for 14 generations resulted in resistance ratios from 10- to >500-fold resistance by using 2 different methods of selection. Partial cross-resistance was exhibited to CryID, CryIE, CryIAb toxins and to the parental strain B. thuringiensis aizawai 7.29. No cross-resistance occurred to CryIF. Resistance declined from >500 times the resistance ratio to >74 times after 1 generation without selection pressure, remained ≈100 times after 3–6 generations and fell to 11 times after 8 generations. Sex ratio remained the same over selection period. Pupal weights of the selected strain varied, but we observed no trend in these differences. Developmental time increased from 5 to 6 d for treated, resistant insects.
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