Split-dose recovery in epithelial and vascular-connective tissue of pig skin

Abstract

In the first 16 weeks after irradiation, two distinct waves of reaction can be observed in pig skin; the first wave (3-9 weeks) represents the expression of damage to the epithelium while the second is indicative of primary damage to the dermis, mediated through vascular injury. Following beta-irradiation with a strontium-90 applicator, a severe epithelial reaction was seen with little subsequent dermal effects. X-rays (250 kV) on the other hand, produced a minimal epithelial response at doses which led to the development of dermal necrosis after 10-16 weeks. Comparison of single doses with two equal doses separated by 24 h produced a D2-D1 value of 7.0 Gy at the doses which produced moist desquamation in 50% of fields (ED50) after strontium-90 irradiation. After X-irradiation comparison of ED50 doses for the later dermal reaction suggested a D2-D1 value of 4.5 Gy. Over this same dose range of X-rays the D2-D1 value for the first wave epithelial reaction was 3.5 Gy. These values of D2-D1 for epithelial and dermal reactions in pig skin were compared with published data and were examined in relation to the theoretical predictions of a linear quadratic model for tissue target cell survival. The results were broadly in keeping with the predictions of such a model.