Abstract
Splenic autotransplantation in tissue is a clinical method for partial preservation of splenic function following total splenectomy. Microangioarchitecture and vascular density of the implants as prerequisites for splenic function and the influence of particle size and fixation on neo-and revascularization have not been analyzed in detail. Homologous spleen particles of different sizes were implanted into transparent dorsal skinfold chambers in 30 golden hamsters. By means of intravital microscopy and computerized video analysis techniques, the process of capillary sprouting could be quantitatively analyzed in the awake animal over a 14-day period following implantation. Measurements included vessel diameter, vascular density, and tissue surface pO2. Based on the results obtained, we suggest limiting the size of splenic autografts to a volume that can be nourished by diffusion until the implants become vascularized. The use of fibrin glue for implant fixation does not interfere with vascularization of the graft.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
